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. 2017 Apr 10;12(4):e0175593. doi: 10.1371/journal.pone.0175593

Fig 3. Endogenous nuclear HIF-2α and B-Myb interact.

Fig 3

(A) Endogenous interaction between HIF-2α and B-Myb. 786-O cell lysates were immunoprecipitated (IP) with an anti-HIF-2α antibody and immunoblotted with anti-B-Myb or anti-HIF-2α antibodies. Representative data from three independent experiments are shown. (B) Cytoplasmic and nuclear fractions were prepared from 786-O cell lysates and utilized for co-immunoprecipitation assays as in (A). Endogenous HIF-2α and B-Myb are enriched in the nucleus but also localize to the cytoplasm. An interaction between HIF-2α and B-Myb was detected in the nuclear fraction but not the cytoplasmic fraction. Hsp90 and Lamin B1 were used as cytoplasmic and nuclear protein markers, respectively. Representative data from two independent experiments are shown.