Fig 4. Lymphoid tissue supports DY PrPSc formation in vitro.
Western blot (A) and quantification (B) of PMCA reactions containing hamster spleen homogenate as the template for conversion were seeded with brain homogenate (lanes 1–8) or detergent enriched, PK digested PrP-res (lanes 9–10) from uninfected (UN), 139H scrapie (139H), HY TME (HY) or DY TME (DY) infected hamsters. Reactions before (-) or after (+) PMCA are shown. The 19 and 21 kDa unglycoslyated PrPSc polypeptides are indicated on the left of the panel.