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. 2017 Mar 27;13(3):e1006701. doi: 10.1371/journal.pgen.1006701

Fig 1. Evidence for DNA inversion at the flagellar switch.

Fig 1

(A) Nucleotide sequences corresponding to the 5’ UTR of the flgB operon from genome sequences available for PCR ribotype 027 strains were aligned using Clustal Omega. Shown are the regions corresponding to the putative flagellar switch and flanking imperfect inverted repeats. For strain BI1 “inverse”, the alignment was repeated after replacing the putative switch with its reverse complement. Identical nucleotides are indicated with blue shading. (B) Diagram of the PCR strategy used to detect the putative flagellar switch orientation. The primer names and sequences used for each strain are listed in the S2 Table. The predicted product sizes are based on R20291 sequence. (C) Orientation-specific PCR products for the flagellar switch from three C. difficile strains representing three ribotypes (R20291, 027, NCBI Accession No FN545816.1; ATCC43598, 017, NCBI sequence read archive SRX656590 [115]; 630Δerm, 012, NCBI Accession No. EMBL:LN614756 [116]).