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. 2017 Mar 27;13(3):e1006701. doi: 10.1371/journal.pgen.1006701

Fig 3. Assessing the purity of enriched populations.

Fig 3

(A, B) Asymmetric PCR-digestion assay of genomic DNA to determine the orientation of the flagellar switch in individual colonies. Bacteria from individual colonies contain the switch predominantly in the published orientation (A) or the inverse orientation (B). Two representatives of each are shown. (C) Quantitative PCR results of the flagellar switch orientation in enriched phase variant populations (published, n = 9; inverse, n = 10). The ΔΔCt method was used to determine the relative DNA copies of the flagellar switch orientation in enriched populations relative to the rpoC gene.