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. 2017 Mar 27;13(3):e1006701. doi: 10.1371/journal.pgen.1006701

Fig 5. Motility medium spatially segregates flagellar phase variant populations.

Fig 5

(A) The motility of C. difficile R20291 flg ON and OFF isolates was evaluated in soft agar medium. A non-motile R20291 sigD mutant was included as a control. (B) The motility of flg ON and OFF isolates was quantified by measuring the diameters every 24 hours for 3 days, and means and standard deviations are shown. * p < 0.05 by t-tests comparing values at each time point. The data are combined from two independent experiments with four biological replicates of each flg phase. (C) At 24, 48 and 72 hours, bacteria were sampled from the center and edges of the growth in the motility assays for flg ON and OFF isolates (top and bottom panels, respectively) and subjected to asymmetric PCR-digestion assays. Black arrows indicate products for the ON orientation; open arrows, the OFF orientation. Shown are representative images from 2 independent experiments, each with two biological replicates of each flg phase.