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. 2017 Mar 27;13(3):e1006701. doi: 10.1371/journal.pgen.1006701

Fig 8. Identification of the recombinase that mediates inversion of the flagellar switch.

Fig 8

(A,B) Orientation-specific PCR (Fig 1B) assays to identify the conserved recombinase that catalyzes inversion at the flagellar switch from ON to OFF (A) and OFF to ON (B). The gene name or R20291 locus tag numbers for the eight conserved recombinases are shown. A 375 bp product indicates the ON orientation; a 281 bp product indicates the OFF orientation. (C) Orientation-specific PCR for the flagellar switch to determine whether the recV cwpV locked ON and OFF mutants were locked for the flagellar switch. (D) Orientation-specific PCR for the flagellar switch in complemented recV mutants (pPtet::recV) and controls.