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. 2017 Mar 27;13(3):e1006631. doi: 10.1371/journal.pgen.1006631

Fig 1. SEPT12S196E/S196E mice displayed abnormal sperm morphology and motility.

Fig 1

(A) Comparison of amino acid sequences flanking the Ser198 analogous sites of SEPT12 in various species using the ClustalW2 program at EMBL-EBI. The bracketed amino acid residues are analogous sites of Ser198. (B) Schematic illustration of the strategy for generating the SEPT12S196E KI allele in embryonic stem cells. (C) Gross morphology of the testis and epididymis from WT and SEPT12 KI mice. Scale bar, 1 cm per unit. (D) Hematoxylin and eosin staining of testicular sections of stage XII tubule (top) and epididymal (bottom) sections from WT and SEPT12 KI mice. Scale bar, 100 μm. (E) Quantitative representation of testis/body weight, sperm counts, abnormal sperm morphology and sperm motility from WT and SEPT12 KI mice (Each genotype, N = 5). The sperm were isolated form the vas deferens. The data are represented as the means ± SEM. ***P<0.001.