Fig 3. The annulus was completely absent in the seminiferous tubules of SEPT12^S196E/S196E mice during spermiogenesis.

(A, B) Testicular sections of WT (A) and SEPT12^S196E/S196E (B) mice were stained to detect the annulus using an anti-SEPT4 antibody (green), the acrosomes using PNA lectin (red) and nuclei using DAPI (blue). Spermatogenetic stages were determined based on the morphology of the acrosome. The annulus is indicated with arrows. S1-16, step 1–16 spermatids. Scale bar: 10 μm.