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. 2017 Mar 27;13(3):e1006631. doi: 10.1371/journal.pgen.1006631

Fig 8. Working models for SEPT12 phosphorylation during sperm annulus biogenesis.

Fig 8

SEPT12 filament is comprised of SEPT12-7-6-2-2-6-7-12 and SEPT12-7-6-4-4-6-7-12 octamers. Phosphorylation on SEPT12 Ser198 through PKA dissociates SEPT12 from the SEPT7-6-2 and SEPT7-6-4 complexes but does not disrupt the SEPT12-SEPT12 interaction. In WT mice, sperm annulus is assembled by SEPT12 filament at sperm neck and migrated along axoneme toward mid-principal piece junction. Disorganization of SEPT12 filament in phospho-SEPT12 KI mice fails to establishment of sperm annulus and lead to sperm deformation. The dotted arrows indicate the elongation of the filament with the addition of the SEPT octamers. NC, the N- and C-termini of the septin proteins. G, the GTP-binding domain of septins.