Fig 8. Activity-induced BDNF expression is affected in Ptpn11^D61Y neurons.

(A) Representative images of neurons in low-density cultures infected with the lentivirus containing the BDNFpI+II EGFP reporter in basal conditions and upon treatment with 4AP/Bic for 30 min. Neurons are stained with antibodies against GFP to enhance the intrinsic EGFP signal, MAP2 as a neuronal marker, and DAPI. (B) Schema of the activity reporter, in which the BDNF promoters I and II drive the expression of EGFP. The cAMP response element (CRE) is depicted. (C) Quantification of the GFP IF that was measured in the nuclei of control and Ptpn11^D61Y neurons. The reporter signal increased significantly upon stimulation in control but not in Ptpn11^D61Y neurons. Data are shown as mean ± SEM and analyzed using one-way ANOVA followed by Bonferroni´s multiple comparison test (*p≤0.05) Scale bar: 10 μm.