Figure 4.

ASA inhibits CF proliferation and apoptosis by impairing autophagy. (A) CFs exposed to hypoxia were treated with 0.5, 2.5, or 5 mmol/L ASA for 12 h, then the changes in LC3-II were detected by Western blot. (B) CFs were treated with 2.5 mmol/L ASA combined with the lysosome inhibitor PepA for 12 h, then the changes of LC3-II, phosphorylated Akt and PARP 1 were all detected by Western blot. (C) CFs were treated with 2.5 mmol/L ASA and the autophagy inducer rapamycin for 12 h, then the proliferation of CFs was analyzed by EdU. Mean±SEM. n=3. ^**P<0.01.