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Double tracer dilution method. The endothelial glycocalyx volume can be obtained by subtracting the circulating volume measured by labeled erythrocytes from the total circulation volume measured using dextran (or other tracer permeable to the glycocalyx layer).
Volume of endothelial glycocalyx = Total circulation volume measured using dextran 40 (A) – Circulation volume measured by labeled RBCs (B)
*RBC, red blood cell
