Table 1. ED50, IC50, p53 mutational status and BMI-1 protein levels in AML cell lines.
| Cell lines | ED50 (nM) | IC50 (nM) | p53 mutation status | BMI-1 levels (MFIR) |
|---|---|---|---|---|
| MOLM-13 | 61.5 | 22.4 | Wild-type | 3.7 |
| OCI-AML3 | 60.3 | 45.6 | Wild-type | 1.0 |
| MOLM-14 | 52.5 | 22.4 | Wild-type | 4.7 |
| MV4-11 | 41.6 | 40.6 | Wild-type | 1.1 |
| U-937 | 55.4 | 27.9 | Mutant | 5.3 |
| HL-60 | 90.4 | 25.1 | Mutant | 0.7 |
| OCI-AML3 ShC | 52.5 | 30.9 | ||
| OCI-AML3 Shp53 | 57.2 | 29.7 | ||
| MOLM-13 ShC | 32.8 | 20.5 | ||
| MOLM-13 Shp53 | 44.7 | 21.4 | ||
| MV4-11 ShC | 57.5 | 41.1 | ||
| MV4-11 Shp53 | 66.3 | 34.5 |
Abbreviations: MFIR, mean fluorescence intensity ratio; ShC, shRNA control. Shp53, p53 shRNA. MFIR was normalized to that of OCI-AML3 cells.