Figure 2.

s-RNYs promote apoptosis and inflammation in lipid-laden macrophages. Cell death data by flow cytometry from human THP1 cells (a) or mouse BMDMs (b) transfected with a cocktail of siRNAs against the terminal loop of all RNYs (siRNYs) to induce s-RNY maturation, a cocktail of 2'-OMe-RNA antisense oligonucleotides (AS) to all s-RNYs expressed, or control. Cells were stimulated with 0.25 mM of PA for 18 h (left panel), 0.25 μM Tg in combination with 100 μg/ml of oxLDL for 48 h (middle panel) or left unstimulated (right panel). Percentage of apoptotic cells was determined by staining with annexin V-FITC. Data are presented as mean and S.D. (n=3). Immunoblot analysis of IκBα, actin, total caspase 3 (casp3) and its cleaved form (c-casp3) in THP1 cells (c) or BMDMs (d) transfected with either a cocktail of AS to all s-RNYs or control. Cells were stimulated with 0.25 mM of PA for 18 h (left panel), 0.25 μM Tg in combination with 100 μg/ml of oxLDL for 48 h (middle panel) or left unstimulated (right panel). Student's t-test: *P<0.05; **P<0.01; ***P<0.001