Figure 3.

Endogenous LncRNA-H19 is required for CSCs maintenance in vivo. (a) H19 depletion attenuated xenograft tumor growth after the first tumor transplantation. MDA-MB-231 cells were infected with lentivirus shH19-3 or NTC; cells were subcutaneously injected into nude mice, respectively. Representative images of subcutaneous tumors taken 6 weeks post inoculation (left) and the tumor growth curves were presented (right). Data are presented as the mean ± S.D. (n = 5; *P<0.05, **P<0.01 and ***P<0.001). (b) H19 knockdown significantly repressed in vivo tumor formation after second serial tumor transplantation. Shown were representative images of subcutaneous tumors collected at end point (left) and summary of tumor xenografts formation of different groups in nude mice (right). (c) The knockdown efficiency of H19 in the second tumor xenografts were detected by RT-qRCR. Error bars represent mean±S.D. of triplicates. (d–f) The cancer cells isolated from H19 knockdown tumor xenografts displayed the reduced clonogenicity (d), migration (e) and mammosphere-forming (f) abilities. Data were shown as mean±S.D. from three independent experiments, **P<0.01 and ***P<0.001, respectively