Figure 2.
Mitochondrial dysfunction in 26S proteasome-impaired neurons. (a) Relative MMP of mitochondria purified from 3, 4 and 6-week-old control and Psmc1fl/fl;CaMKIIα-Cre cortices using JC1 potentiometric dye. MMP is calculated as the ratio red:green fluorescence. Protonophore carbonyl cyanide m-chlorophenyl hydrazine (CCCP) was used as a control; n≥3 mice. (b) Relative complex I activity of mitochondria purified from 3, 4 and 6-week-old control and Psmc1fl/fl;CaMKIIα-Cre cortices. Rotenone was used as a control; n≥8 mice. Error bars represent S.E.M. in (a) and (b). **P<0.01 by unpaired Student's t-test