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. 2017 Jan 5;8(1):e2537. doi: 10.1038/cddis.2016.453

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a-f) Isolation and characterization of RPE from donors. The isolated RPE from AMD and control donors express RPE-specific proteins ZO-1, Bestrophin and CRALBP. A representative image of immunostaining is shown for each group. Bar represents 100 μM. (g) Gene expression analysis by qRT-PCR confirming the expression of RPE marker genes in the RPE isolated from donors (controls 6, 10, 23 and 25; AMD 9, 17, 19 and 32). (h) Gene expression analysis of AMD-associated genes in AMD and normal RPE. **P<0.01, ****P<0.0001