Figure 1.

miR-17-5p activates 3T3-L1 adipogenic differentiation. (a-b) HFD feeding (12 weeks) increased the level of miR-17-5p but not levels of other miR-17-92 cluster members in mouse epididymal fat tissue. (c) Illustration of the differentiation procedure with miR-17-5p inhibition. (d) Oil red O staining. (e) Semi-quantitative assessment of Oil red O content. (f) Triglyceride levels in differentiated 3T3-L1 cells with and without miR-17-5p inhibition. (g) Illustration of the differentiation procedure with miR-17 overexpression. (h) Oil red O staining. (i) Semi-quantitative assessment of Oil red O content. (j) Triglyceride levels in differentiated 3T3-L1 cells with and without miR-17 over-expression. (k-l) Detection of expression of genes that encode five adipogenic differentiation markers with and without miR-17-5p inhibition (k), as well as with and without miR-17 over-expression (l). N> or=3 for panels A, B, D–F and H–L. *P<0.05; **P<0.01; ***P<0.001. CON, control; miR-17, miR-17-5p