Figure 2.

Tcf7l2 level is negatively regulated by miR-17-5p in 3T3-L1 cells. (a-d) miR-17 over-expression repressed, while miR-17-5p inhibition increased Tcf7l2 levels. (e-f) miR-17 over-expression generated no effect on Tcf7 or Tcf7l1 expression (e), while its inhibition reduced Tcf7l1 expression (f). (g) miR-17 over-expression repressed Tcf7l2 protein levels. The middle and right panels show the results of densitometric analyses. (h) miR-17-5p inhibition increased Tcf7l2 protein levels. For (g) and (h), middle and right panels show the results of densitometric analyses. (i) Illustration of the potential miR-17-5p binding site on Tcf7l2 3′UTR. (j) Decreased LUC activities were observed in cells co-transfected with miR-17 and Luc-Tcf7l2, which was reversed when the miR-17-5p binding site was mutated. N> or =3 for panels (a–h) and (j). *P<0.05; **P<0.01; ***P<0.001. CON, control; miR-17, miR-17-5p