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. 2017 Jan 5;8(1):e2538. doi: 10.1038/cddis.2016.458

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Hb increases susceptibility to cell death in cellular model of PD. Differentiated Hb cells (Hb) and control cells (control) were treated with MPP⁺ (a–c) or rotenone (d–f) at the indicated concentrations for 16 h. (a and d) Western blotting analysis of cleaved Caspase-3 (cleaved Casp3) expression. α- and β-Globins were detected with anti-FLAG and anti-MYC antibodies, respectively. For normalization, the levels of pro-Caspase-3 (pro-Casp3) were detected. β-Actin was used as a loading control. (n=3, n=3) (b and e) FACS analysis. Percentage of subG1 cells is expressed as fold increased relative to untreated cells, arbitrary set to 1. (n=3, n=4) (c and f) WST-1 analysis. In graph are represented the percentage of viable cells relative to untreated cells, arbitrary set to 100%. (n=4, n=3) Values are mean±S.D. Data were evaluated statistically by Student's t-test. Resulting P-values are indicated (NS=not significant)