Figure 5.

AAV9-mediated delivery of Hb in SNpc inhibits improvement of motor performance and triggers Hb aggregates in DA cells. (a) Scheme of experimental settings. (b) Latency in rotarod test in AAV9-control (n=18) and AAV9-Hb (n=19) mice at 5, 10, 15 and 20 r.p.m. (c) Immunohistochemistry of coronal sections of AAV9-control and AAV9-Hb mouse brain. TH⁺ fibres in the striatum were stained with anti-TH antibody. Scale bar 800 μm. (d) Immunohistochemistry of coronal sections of AAV9-Hb and AAV9-control mouse brain. SN was stained with anti-TH antibody. Scale bar 200 μm. (e) Densitometric analysis of TH⁺ fibres in AAV9-Hb (n=18) and AAV9-control (n=18) mice. Values are expresses as a percentage relative to the contralateral side of AAV9-control mice, arbitrary set to 100%. (f) Quantitative analysis of TH⁺ cells number in AAV9-Hb (n=16) and AAV9-control (n=15) mice. Values are expressed as the number of total TH⁺ cells relative to the contralateral side of AAV9-control mice. (g) Immunohistochemistry of coronal sections of AAV9-Hb mouse brain. SN was stained with anti-TH antibody. Infected cells were stained with anti-FLAG (α-globin) antibody. Arrow indicates α-globin aggregates. Nuclei were visualized with DAPI (4,6-diamidino-2-phenylindole). Scale bar 50 μm. (h) Immunohistochemistry of coronal sections of AAV9-Hb mouse brain. SN was stained with anti-TH antibody. Infected cells were stained with anti-FLAG (α-globin) and anti-MYC (β-globin) antibodies. Arrow indicates globin aggregates. Scale bar 10 μm. All data are represented as mean±S.E.M. Data were evaluated statistically by one-way analysis of variance. Resulting P-values are indicated