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. 2017 Jan 5;8(1):e2539. doi: 10.1038/cddis.2016.468

Figure 5.

Figure 5

miR-224 mediates the oncogenic potential of TAZ in OS cells in vitro. (a) MTT analyses of the indicated cells, including control cells and TAZ shRNA knockdown cells transfected with the miR-224 mimic or negative control. Each bar represents the mean±S.D. of three independent experiments. (b) Cell apoptosis of MG-63 and U2OS cells co-transfected with pre-miR-224 and shTAZ was detected by Annexin V–PE staining. (c and d) The effect of miR-224 on migration and invasion was hampered by shTAZ. Transwell assays were performed to detect the cell migration and invasion of MG-63 and U2OS cells co-transfected with pre-miR-224 and shTAZ, compared with controls. (e) Soft agar colony formation ability was detected in MG-63 cells co-transfected with pre-miR-224 and shTAZ. Colonies were stained with crystal violet, photographed and quantified by using ImageJ (details are shown in the inserts; n=4, mean±S.D., *P<0.01). (f) Overexpression of miR-224 promoted the growth of TAZ knockdown cells, as determined by colony formation assays, Error bars represent the mean±S.D. from three independent experiments; *P<0.01