Figure 2.

Effects of cysteamine on internalization and clearance of P. aeruginosa-O1 (PAO1) in bone marrow-derived macrophages (BMDMs) from wild-type (WT) and Cftr^{F508del/F508del} mice. BMDMs from Cftr^{F508del/F508del} (a–c) or WT (d–f) mice treated with 250 μM cysteamine (n=10) or vehicle (n=10) for 10 min before infection followed by 10 min (a–b, d–e) or 3 h (c, f) of gentamicin culture. P. aeruginosa internalization expressed as number of CFUs of PAO1 (a, d) or enumerated by immunofluosescence (b, e) after 10 min of gentamicin culture (***P<0.001 versus vehicle; Student's t-test); (c, f) clearance of P. aeruginosa expressed as percentage of living bacteria after 3 h of culture respect to the total amount of internalized bacteria (***P<0.001 versus treatment with vehicle; Student's t-test). Means±S.D. of three independent measurements for each group of experiments. Asterisks indicate significant differences