Figure 4.

Cysteamine does not restore defective killing of P. aeruginosa by bone marrow-derived macrophages (BMDMs) from Cftr^{F508del/F508del}/Becn1^+/− mice. BMDMs from wild-type (WT) (n=10) (a–c), Becn1 haplo-insufficient (Becn1^+/−) (n=3) (a–c), Cftr^{F508del/F508del} (n=10) and Cftr^{F508del/F508del}/Becn1^+/− (n=3) (d–f), mice infected with P. aeruginosa-O1 (PAO1) for 10 min followed by 10 min (a, d), or 3 h (b–c and e–f) of culture with gentamicin. Number of CFUs of PAO1 after 10 min (a, d) or 3 h (b, e); clearance of PA expressed as percentage of living bacteria after 3 h of culture respect to the total amount of internalized bacteria (c, f). Effects of the pre-treatment with cysteamine in BMDMs from Becn1^+/− (a–c) and Cftr^{F508del/F508del}/Becn1^+/− (d–f) mice. Means±S.D. of three independent measurements for each group of experiments. *P<0.05, **P<0.01, Student's t-test