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. 2017 Jan 12;8(1):e2546. doi: 10.1038/cddis.2016.478

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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CRB3 stabilizes the Kibra protein by inhibiting its degradation, and Kibra rescues the changes in cell proliferation induced by CRB3 deregulation. (a) MCF10A cells were treated with cycloheximide (100 μg/ml) and MG132 (5 μM) for the indicated times. Cell lysates were examined for the levels of the FRMD6 and Kibra proteins by immunoblotting. (b) T47D cells were treated with cycloheximide (100 μg/ml) and MG132 (5 μM) for the indicated times. The levels of the FRMD6 and Kibra proteins were determined by immunoblotting. (c, d) MCF10A cell lysates were subjected to immunoprecipitation experiments with an anti-CRB3 or anti-Kibra antibody. Immunoblot analyses were conducted for the indicated proteins. (e, f) Immunoprecipitation experiments were performed in T47D cells. (g, h) The protein levels were detected by immunoblotting. (i, j) DNA synthesis was examined in MCF10A cells using BrdU incorporation. ***P<0.001. Data are mean±S.D. (k and l) Aberrant formation of acini was detected in 3D cultures of MCF10A cells. Scale bar, 50 μm.***P<0.001. Data are mean±S.D. (m, n) BrdU incorporation was assessed to examine DNA synthesis in T47D cells. *P<0.05, ***P<0.001. Data are mean±S.D.