Figure 5.

Simultaneous CHIP overexpression and DNA damage substantially enhances the growth inhibition of cancer cells. (a) Effects of CHIP overexpression on the growth inhibition of cells treated with CPT. Growth inhibition of cancer cells by transient overexpression of CHIP was determined in the presence of CPT. Perifosine was used as a negative control. The values represent the mean ± SEM of three independent experiments with triplicate measurements. **P<0.01 vs Con. (b) Comet assay of cells overexpressing HA-CHIP on CPT exposure. The comet assay was performed to visualize DNA damage of the cells transfected with HA-CHIP on CPT exposure. Perifosine was used as a negative control. Scale bar indicates 200 μm. (c) Apoptosis analysis of cells transiently overexpressing CHIP on CPT exposure. Immunoblot analysis was performed using antibody of PARP1and γ-H2AX