Figure 4.

ATG5-ATG12 positively regulates FMDV-induced IFN-β and IL-6. (a) Overexpression of ATG5-ATG12 enhances IFN-β production at mRNA and protein level. PK-15 cells were transfected with vector, Flag-ATG5 and Flag-ATG12 plasmid for 24 h, followed with FMDV infection (MOI=1). Total RNAs were extracted at indicated time points, and IFN-β mRNA was quantified with quantitative real-time RT-PCR analysis. The culture medium was collected at indicated time points for quantification of the secretion of IFN-β with ELISA. (b) Overexpression of ATG5-ATG12 enhances IL-6 production at mRNA level and protein level. The experiments were performed similarly to (a). The level of IL-6 mRNA and the secretion of IL-6 were detected with quantitative real-time RT-PCR analysis and ELISA, respectively. (c) Knockdown of ATG5-ATG12 reduces IFN-β production at mRNA level and protein level. PK-15 cells were transfected with control siRNA (NC) or ATG5-ATG12 siRNA for 36 h, followed with FMDV infection (MOI=1). The level of IFN-β mRNA and the secretion of IFN-β were detected with quantitative real-time RT-PCR analysis and ELISA, respectively. (d) Knockdown of ATG5-ATG12 reduces IL-6 production at mRNA level and protein level. The experiments were similarly performed as (c). The level of IL-6 mRNA and the secretion of IL-6 were detected with quantitative real-time RT-PCR analysis and ELISA, respectively. Above data are representative of three independent experiments. Graphs show mean±S.D.; n=3. *P<0.05