Figure 6.

ATG5-ATG12 positively regulates IRF3 signaling during FMDV infection. (a) Overexpression of ATG5-ATG12 promotes the IRF3 signaling during FMDV infection. PK-15 cells were transfected with vector, Flag-ATG5, and Flag-ATG12 plasmid for 24 h, followed with FMDV infection. Cells were harvested at 0, 1, 3, 5, and 7 h.p.i. The levels of TRAF3, phospho-TBK1, IRF3, phospho-IRF3, Flag-ATG5-ATG12, ATG5-ATG12, and viral proteins were analyzed with Western blot. (b) Knockdown of ATG5-ATG12 reduces the IRF3 signaling during FMDV infection. PK-15 cells were transfected with control siRNA (NC), or ATG5-ATG12 siRNA for 36 h, followed with FMDV infection. Cells were harvested at 0, 1, 3, 5, and 7 h.p.i. The levels of TRAF3, phospho-TBK1, IRF3, phospho-IRF3, ATG5-ATG12, and viral proteins were analyzed with Western blot. Above experiments were performed in triplicates