Table 2. Conservation across species of amino acids in missense mutations.

Human	A38	S39	Y40	A41	R42	K43	W44	Q45	Q46	M47	R48	P49	I50	P51	T52
CYP4V2 c.134A>C, p.Q45P
Chimp	A	T	Y	A	R	K	W	Q	Q	Q	R	P	I	P	T
Mouse	V	S	Y	A	R	K	W	Q	Q	Q	R	S	I	P	S
Rabbit	A	S	Y	A	R	K	W	Q	Q	Q	R	P	I	P	T
Cow	A	S	Y	A	R	K	W	R	Q	R	R	P	V	P	T
Horse	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Dog	A	S	Y	A	Q	K	C	H	Q	H	R	A	F	P	S
Chicken	L	N	Y	W	W	W	W	W	V	W	K	P	I	P	G
Zebrafish	L	S	Y	M	M	W	W	K	E	K	K	P	I	P	G
CYP4V2 c.1178C>T, p.P393L
Human	K386	E387	T388	L389	R390	L391	F392	P393	S394	V395	P396	L397	F398	A399	R400
Chimp	K	E	T	L	R	L	F	P	S	V	P	L	F	A	R
Mouse	K	E	T	L	R	V	F	P	S	V	P	L	F	A	R
Rabbit	K	E	T	L	R	L	F	P	S	V	P	L	F	A	R
Cow	K	E	S	L	R	L	F	P	S	V	P	F	F	A	R
Horse	K	E	T	L	R	L	F	P	S	V	P	L	F	A	R
Dog	K	E	S	L	R	L	F	P	S	V	P	L	F	A	R
Chicken	K	E	A	L	R	L	F	P	S	V	P	M	F	A	R
Zebrafish	K	E	S	L	R	L	F	P	S	V	P	F	F	A	R
CYP4V2 c.1355G>A, p.R452H
Human	F445	P446	E447	N448	A449	Q450	G451	R452	H453	P454	Y455	A456	Y457	V458	P459
Chimp	F	P	K	N	A	Q	G	R	H	P	Y	A	Y	V	P
Mouse	F	P	E	N	S	Q	G	R	H	P	Y	A	Y	V	P
Rabbit	F	P	E	N	A	Q	G	R	H	P	Y	A	Y	V	P
Cow	F	P	E	N	L	K	G	R	H	T	Y	A	Y	V	P
Horse	F	P	E	N	S	Q	G	R	H	P	Y	A	Y	V	P
Dog	F	P	E	N	L	Q	G	R	H	P	Y	A	Y	I	P
Chicken	F	P	E	N	S	K	G	R	H	P	Y	A	Y	V	P
Zebrafish	L	P	E	N	C	V	G	R	H	P	Y	A	Y	I	P
CYP4V2 c.71T>C, p.L24P
Human	G17	A18	A19	S20	A21	L22	S23	L24	A25	G26	A27	S28	L29	V30	L31
Chimp	G	A	A	S	A	V	S	L	A	G	A	S	L	V	L
Mouse	G	A	A	S	A	V	S	L	A	G	A	T	I	L	I
Rabbit	G	A	A	S	A	V	S	L	A	G	A	S	L	F	L
Cow	S	G	L	C	A	V	S	L	A	G	A	T	L	T	L
Horse	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
Dog	G	A	L	G	F	P	S	L	G	G	P	P	L	V	V
Chicken	V	A	G	A	I	T	S	L	A	A	D	G	V	T	V
Zebrafish	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–

Alignment of novel mutations (bold) identified in the CYP4V2 gene in nine species ranging from human to zebrafish.