Figure 1.

MDA-MB-231br cells are strongly capable of invasion and migration. (A) The novel human breast cancer cell line MDA-MB-231br isolated from the metastatic mouse model and MDA-MB-231 were stimulated to migrate and invade in Transwell chambers. Representative photographs (upper panel) and quantification (lower panel) are shown. Columns: average of 3 independent experiments, * p<0.05. Magnification ×100. (B) EMT markers, including E-cadherin, vimentin, ezrin, cortactin, annexin II, hsp27, lasp2, and ezrin-T567 were differently expressed in MDA-MB-231 and MDA-MB-231br cell lines, according to Western blotting analysis with GAPDH as the internal control. (C) Differentially expressed proteins were identified in MDA-MB-231 and MDA-MB-231br cell lines by two-dimensional electrophoresis (2-DE) and mass spectrometry.