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. 2017 Apr 1;23:1583–1596. doi: 10.12659/MSM.904124

Figure 2.

Figure 2

Downregulation of ezrin reverses EMT in MDA-MB-231 and SUM1315 cells. (A) The expression levels of ezrin were verified in MDA-MB-231 and SUM1315 after transfection of shRNA against ezrin or upon ezrin overexpression, by Western blot and qRT-PCR, respectively. ** p<0.01, *** p<0.001. The phosphorylation of ezrin at position Y-567 was decreased with decreased ezrin expression. (B) The expression of ezrin was verified in MCF-7 and ZR-75-1 cells after ezrin overexpression, by Western blots and qRT-PCR, respectively. ** p<0.01, * p<0.05. (C) Western blot analysis revealed that knockdown of ezrin resulted in increased expression of an epithelial marker (E-cadherin) and decreased expression of a mesenchymal marker (α-SMA) in MDA-MB-231 and SUM1315 cells. Snail, Twist, and Twist2, which are key transcription factors that promote EMT, were significantly decreased in ezrin-downregulated MDA-MB-231 and SUM1315 cells. (D) The overexpression of ezrin did not alter the expression of E-cadherin and α-SMA in MCF-7 and ZR-75-1 cells.