Figure 5.

Ezrin and cortactin express a similar pattern in breast cancers. (A) In MDA-MB-231 or SUM1315 cells, the reduction in ezrin expression occurred alongside the reduction in cortactin. (B) Co-IP analysis suggested a connection between ezrin and cortactin in SUM-1315 cells. (C) The expression and phosphorylation of ezrin did not change in MDA-MB-231 or SUM1315 cells transfected with siRNA against cortactin. Knockdown of cortactin resulted in increased expression of E-cadherin and decreased expression α-SMA in SUM1315 cells. (D) Immunohistochemistry revealed significant correlations between ezrin expression and cortactin expression. Magnification ×400.