Figure 1.

Star-PAP is downregulated in breast cancer. (a) Protein levels of Star-PAP in a panel of breast cancer cell lines were detected by western blot. β-Tubulin was used as the loading control. (b) mRNA levels of Star-PAP were quantified by qPCR and normalized to MCF10A cells. Data from triplicate experiments were presented as mean±S.D. (c) Star-PAP mRNA was examined by RT-PCR and the products were analyzed in agarose gel. GAPDH was used as the loading control. (d) Expression levels of Star-PAP in 51 breast cell lines were analyzed using the Neve cell line data set. Data set accession number and P-value are shown. (e–g) Box plots show Star-PAP expression levels in human breast cancer tissues. Data sets for ductal carcinoma in situ (e), invasive ductal carcinoma (f) and invasive breast carcinoma stroma (g) were analyzed. Data set accession number and P-value are shown