Figure 4.

MAPK signaling participated in regulating the MCM7–cyclin D1 signaling in HCC cells. (a) Correlation of MCM7 with CCND1 gene expression in three HCC cell lines. (b) Correlation of MCM7 with 12 MAPK pathway member gene expression. (c) Heat map of changes in the expression of 12 MAPK pathway member genes after lentiviral vector transduction. Western blot analysis (d) and corresponding bar graphs (e) validated the alteration in the activity of three major MAPK members (ERK, JNK and p38) in HCC cells. (f) Correlation of CCND1 with 12 MAPK pathway member gene expression. (g) Heat map of changes in the expression of CCND1 after pretreatment with SB203580 (p38 inhibitor) or U0126 (ERK inhibitor) at different time points (3, 6, 12 and 24 h).; (h) Western blot analysis for cyclin D1 expression level in HCC cells pretreated with SB203580 (for 6 h) or U0126 (for 24 h). Data are represented as mean±S.E.M. from three independent experiments. *P<0.05, **P<0.01, ***P<0.001