Figure 4.
Pericellular D-peptide nanofibrils affect the expression of death receptors and selectively inhibit MDR cancer cells for combination therapy. Western blot shows change of relative amount of cell death receptors and two down-stream proteins over time in (a) MES-SA/Dx5, (b) T98G, (c) A2780-cis, or (d) HS-5 cells treated by pDTP. (e) Relative cell viability of the co-culture of A2780-cis and HS-5, A2780-cis, and HS-5 cells incubated with cisplatin (CDDP) at the concentration of 6 μg/ml in DMEM. (f) Relative cell viability of A2780-cis cells incubated with 216 μg/ml pDTP, 15 μg/ml CDDP, or the mixture of 216 μg/ml pDTP and 15 μg/ml CDDP for 24 h. Cells are pretreated with CDDP for 12 h. Relative cell viability of HeLa cells incubated with (g) pDTP of different concentrations and 10 or 20 μM BAY 11-7085 or 500 nM BTZ; (h) BAY of different concentrations and 100 μM pDTP. The incubation time is 48 h. The initial number of cells is 1.0 × 104/well. n=3. Data are shown as mean±S.D. *P<0.05, **P<0.01 by Student's t-test