Figure 3.

Representative immunofluorescence evidence for co-localization of PAR2/ERK/VEGFR2. Confocal immunofluorescence analysis showing diminutive areas of co-localized DNA in blue, TRYP in red and PAR2 in green, the overlap of TRYP and PAR2 (yellow staining in the merged image) indicates trypsin does co-expresses with PAR2 in the pancreatic tissues with CALCB_p.S30P mutations (a) and that of wild-type (b). Confocal immunofluorescence analysis showing small areas of co-localized DNA in blue, ERK in red and PAR2 in green, overlap of ERK and PAR2 (yellow staining in the merged image) in the endothelial and smooth muscle layers, indicates decreased ERK and PAR2 activity in CALCB_p.S30P mutations (c) than wild-type (d). Confocal immunofluorescence analysis showing small areas of co-localized DNA in blue, AKT in red and PI3K in green, in the endothelial and smooth muscle layers, indicates, double staining of AKT with PI3K showed that AKT co-expresses with PI3K in the tissues with CALCB mutation (e) and wild-type (f). Confocal immunofluorescence analysis showing diminutive areas of co-localized DNA in blue, ERK in red and VEGFR2 in green, indicates littler co-expresses of ERK and VEGFR2 in the tissues with CALCB_p.S30P mutation (g), while it does co-expresses in the wild-type (h). Original magnification: × 400. WT denotes wild-type