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Representative images showing the localization of βCGRP with ER and Golgi in HEK293. Immunofluorescence staining of HEK293 cells with 4′, 6-diamidine-2-phenylidole dihydrochloride (DAPI), antibodies against Flag-tagged wild-type and mutant CALCB (red in (a)) and green in (b), ER (green in (a)), and Golgi (red in (b)). The yellow areas indicate the merging of CALCB and ER or Golgi straining in the podocytes
