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. 2017 Feb 9;8(2):e2612. doi: 10.1038/cddis.2017.38

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Luteolin selectively induced apoptosis in the drug-resistant GC cells. (a) Molecular structural formula of luteolin. (b) UV absorption measured by HPLC shown the purity of luteolin. (c and d) Various gastric tumor cells were treated with fold diluted DDP or luteolin. Cell viability was determined by a MTS method. IC₅₀ value was determined to evaluate the inhibition of tumor cell growth by luteolin. The value of each cells was the mean value of three independent experiments. (e) SGC7901/DDP and HGC27 cells were treated with 15 or 5 μM luteolin and then stained with propidium iodide and AnnexinV-FITC for detecting the apoptosis by flow cytometry. (f) The columns showed the statistical FACS result of three independent experiments, *P<0.05