Figure 2.

The antitumor effect of luteolin was correlated with STAT3 inhibition. (a) Different GC cell lines were subjected to western blot for measuring protein levels of phosphorylated STAT3. (b) The SGC7901/DDP cells were treated with 10 μM luteolin and then were subjected to western blot for measuring protein levels by indicated antibodies. (c and d) SGC7901/DDP cells and HGC27 cells were treated with indicated concentrations of luteolin and then were subjected to western blot for measuring protein levels by indicated antibodies. (e and f) Total RNA of luteolin treated SGC7901/DDP and HGC27 tumor cells was extracted and a QPCR method was adopted to detect the transcription level of indicated genes. The result was obtained of three independent experiments. *P<0.05. (g) SGC7901 and SGC7901/DDP cells were incubated with DCFDA for 30 min, and the fluorescence levels were analyzed by flow cytometer. (h) Western blot analyses of pY-STAT3 level of antioxidants and luteolin treated SGC7901/DDP cells. (i) SGC7901/DDP tumor cells were treated with fold diluted drugs. Cell viability was determined by MTS