Figure 3.

The inhibition of STAT3 by luteolin was SHP-1 dependent. (a) SGC7901/DDP cells treated with luteolin were subjected to western blot for measuring protein levels by indicated antibodies. (b) SGC7901/DDP cells were transfected with PIAS3 and SHP-2 siRNA or control siRNA and then incubated with (+) or without (−) luteolin. Western blotting with indicated antibodies was performed. (c) SGC7901/DDP and HGC27 cells were transfected with SHP-1 siRNA or control siRNA and incubated with (+) or without (−) luteolin. Western blotting with indicated antibodies was performed, respectively. (d) Different gastric tumor cell lines were subjected to western blot for measuring protein levels of gp130. (e) The total RNA of above-treated cells was extracted, and a QPCR method was adopted to test the transcription level of indicated genes. The result was obtained of three independent experiments. *P<0.05. (f) The viability of above treated cells was determined by MTS method. The result was obtained of three independent experiments. *P<0.05. (g) The above-treated cells was stained with propidium iodide and AnnexinV-FITC for detecting the apoptosis by flow cytometry. The right panel shown the static result of three independent experiments.P<0.05 (compare with control), ^#P<0.05 (compared with luteolin only group)