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. 2017 Feb 9;8(2):e2613. doi: 10.1038/cddis.2017.3

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Morphological characteristics and autophagic markers in ovaries and oocytes at 0–4 d.p.p. (a) An autophagosome (arrow) in the oocyte cytoplasm. TEM, scale bar: 200 nm. (b) Numerous mitochondria (arrows) in the oocyte cytoplasm. Lipid droplets (arrows) in the cytoplasm of a somatic cell around oocytes. TEM, scale bars: 1 μm. (c) WB for LC3-1 to LC3-2 in ovaries of 0–4 d.p.p. During autophagy, cytosolic LC3-1 is conjugated to phosphatidylethanolamine to form LC3-phosphatidylethanolamine conjugate (LC3-2), which is recruited to autophagosomal membranes. (d) IF for LC3B (red) and STAT3 (green) in 3 d.p.p. ovaries