Figure 7.

ΔΨm collapses after OGD/zVAD insult in OPCs. (a) Representative photomicrographs of JC-1 staining and quantification of the JC-1 fluorescence ratio. The JC-1 fluorescence ratio was calculated using the average red/green optical density ratio. A low ratio represents a dissipation of ΔΨm. OGD/zVAD led to dissipation of ΔΨm in OPCs 24 h after OGD. RIPK3 inhibition via siRNA attenuated the dissipation of ΔΨm and enhanced OPCs survival 24 h after OGD. Data are presented as means ±S.E.M. from three independent experiments. N=6 for each group in each experiment (scale bar=40 μm) (P<0.05, comparing the siRNA group with the OGD/zVAD group). S: sham; O/z: OGD/zVAD; Si: RIPK3 siRNA; Sc: RIPK3 siRNA scramble. (b) KN-93 inhibits CaMKIIδ phosphorylation in OPCs in the OGD/zVAD group 12 h after OGD, attenuates the dissipation of ΔΨm 24 h after OGD, and enhances OPCs survival 24 h after OGD. Data are presented as means±S.E.M. from three independent experiments. N=6 for each group in each experiment. (Scale bar=40 μm) (P<0.05, comparing the KN-93 group with the OGD/zVAD group). S, sham; O/z, OGD/zVAD; KN-93, CaMKII inhibitor; KN-92, negative control for KN-93