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. 2017 Feb 9;8(2):e2600. doi: 10.1038/cddis.2017.9

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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RASAL2 inhibits EMT of BCa cells. (a) Morphological change of 5637 cells transfected with RASAL2 shRNAs and scramble shRNA. The scale bar is 50μm. Representative pictures and quantification analysis of migration and invasion assays in 5637 cells transfected with RASAL2 shRNAs and scramble shRNA. The scale bar is 100 μm, **P<0.01, ***P<0.001 versus control. (b) Western blotting analysis of EMT markers (E-cadherin, vimentin and ZEB1) in 5637 sublines. (c) Representative pictures and quantification analysis of migration and invasion assays in T24 cells infected with RASAL2 lentivirus and scramble control. The scale bar is 100 μm, **P<0.01, ***P<0.001 versus control. (d) Western blotting analysis of EMT markers (E-cadherin, vimentin and ZEB1) in T24 sublines. GAPDH was used as internal loading control