Figure 3.

Neuronal Atg7 deficiency reduces microglia activation in the dentate gyrus. (a) Representative Iba-1 immunostaining in the dentate gyrus. (b) Representative morphology of Iba-1-positive cells indicating ramified (surveillance microglia), hyper-ramified (intermediate), and unramified (bushy or amoeboid) microglia. (c) Iba-1-positive cells were significantly increased in the SGZ of the dentate gyrus in both WT and KO mice after irradiation. (d) Quantification of Iba-1-positive cells according to morphology in the SGZ of the dentate gyrus at 6 h after irradiation; n=7 per group. *P<0.05, **P<0.01, and ***P<0.001