Figure 3.

The mechanism of CYD-6-17 in inhibiting Wnt pathway. (a) 786-0 KD and HK-2 KD cells were treated with control (Con) or CYD-6-17 for 24 h, the total RNA were subjected to Affymetrix GeneChip cDNA array and heat map were depicted for the relative gene expression (fold changes) normalized with Con. Asterisks indicated Wnt-related effectors. (b) Western blot analyses of the expression of GSK3β and β-catenin in RCC cells treated with CYD-6-17. (c) The effect of CYD-6-17 on β-catenin-mediated gene transcriptional activity in RCC cells transfected with TOP luciferase reporter gene. After normalizing with Renilla luciferase activity, the relative reporter gene activity in each cell was calculated based on the control. (d) Determination of cell cycle regulatory factors in Sor001 cells transfected with vector control, WT, or CA β-catenin expression vector for 24 h (left panel) and then treated with CYD-6-17 for 48 h to determine the cell viability using MTT assay (right panel). The growth inhibition rate was calculated based on the control. *P<0.05