Figure 5.

miR-199a-3p reduces secretion of HGF from LX2 cell line to inhibit its paracrine effect on cancer cells. (a) 3′-UTR-reporter-luciferase assay of HGF in HepG2 cells transfected with vector and premiR-199a-3p. LX2 cells were transfected with vector, pBABE-puro HGF, HGF-shRNA and premiR-199a-3p. Intracellular HGF expression was determined by (b) western blot and secreted HGF by ELISA in (c) cell culture media and (d) serum of HCC patients. (e) Migration and (f) invasion ability of SNU449 cells in the presence or absence of HGF secretion from LX2 was determined. *P<0.05, **P<0.01 and ***P<0.005