Figure 1.

Dose- and time-dependent effect of BAY and medium refreshment on cell viability. (a) Dose-dependent effect of BAY (N=2, n=4) on the viability of G361 and SK-MEL-28 cells (at 72 h). A Boltzmann equation was used to determine the IC₅₀ (x₀) value: y=(A₂+(A₁−A₂)/(1+exp((x−x₀)/dx))). (b) G361 and SK-MEL-28 melanoma cells were treated with 10 nM BAY (N=3, n=6) and their viability was measured at different time points. A sigmoidal (logistic) equation was used to determine the T_1/2 (x₀) value: y=([(A₁−A₂)/(1+(x/x₀)]+A₂). Statistics: In panel b, significant differences with the SK-MEL-28 cell line are indicated by *P<0.05 and ***P<0.001