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. 2017 Mar 30;8(3):e2714. doi: 10.1038/cddis.2017.134

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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miR-491-5p inhibits cell proliferation and promotes apoptosis in gastric cancer cells. (a) qRT-PCR was performed to examine miR-491-5p expression in 22 paired human gastric cancer and adjacent normal tissues. The expression of miR-491-5p was normalized to U6. (b) qRT-PCR analysis of miR-491-5p expression in normal gastric mucosal and gastric cancer cells and normalized against U6 RNA. (c) The effects miR-491-5p on gastric cancer cell proliferation were determined by MTT assay after transfection of miR-491 or control vector at 24, 48, and 72 h. (d) Representative results of a colony formation assay for MKN45/SGC-7901 cells after miR-491 transfection. (e) Apoptosis was detected by annexin-V/propidium iodide combined labeling flow cytometry in MKN45/SGC-7901 cells 48 h after transfection with miR-491 or control vector. Apoptotic evaluation was carried out by calculating the percentage of apoptotic cells. (f) MKN45/SGC-7901 cells were transfected with miR-491 vector and control vector. After 48 h, cell cycle distribution was analyzed by flow cytometry. A histogram indicates the percentage of cells in G0/G1, S, and G2/M cell cycle phases. (g) The expression of pro-caspase 3, active caspase 3, cleaved PARP, BCL-2, CDK2, CCNA2, and β-catenin was analyzed by western blot (*P<0.05, **P<0.01)