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. 2017 Mar 30;8(3):e2714. doi: 10.1038/cddis.2017.134

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Expression levels of Wnt3a affect gastric cancer cell progression. (a) The expression levels of Wnt3a were measured by qRT-PCR (upper panel) and western blot (lower panel) in MKN45/SGC-7901 cells transfected with siWnt3a. (b) MTT assay was performed to determine the growth of gastric cancer cells treated with siWnt3a or a negative control (si−ctrl). (c) The colony formation assay was performed several days after transfection of gastric cancer cells with siWnt3a or a negative control (si−ctrl). (d) Apoptosis was determined in gastric cancer cells at 48 h after transfection with siWnt3a. (e) Cell cycle distribution was determined in gastric cancer cells 48 h after transfection with siWnt3a by propidium iodide staining and flow cytometry. The histogram indicates the percentage of cells in G0/G1, S, and G2/M cell cycle phases. (f) Protein expression of pro-caspase 3, active caspase 3, cleaved PARP, BCL-2, CCND1, and β-catenin in gastric cancer cells transfected with siWnt3a or si−ctrl was analyzed by western blot. (g) The expression levels of Wnt3a were determined by qRT-PCR in MKN45/SGC-7901 cells transfected with Wnt3a expression vector. (h) MTT assay was performed to measure the impact of gastric cancer cells treated with miR-491 plus Wnt3a expression vectors or related negative controls. (i) Cell apoptosis were performed to determine the effect of gastric cancer cells treated with miR-491 plus Wnt3a expression vectors or related negative controls (*P<0.05, **P<0.01)