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. 2017 Mar 30;8(3):e2710. doi: 10.1038/cddis.2017.135

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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P65 inhibition by siRNA influences cell proliferation, apoptosis, and inflammation in vitro. (a) Fbs proliferation. (b) The apoptosis of Fbs by flow cytometry. The data are shown as the means±S.D. Statistical significance was calculated by one-way ANOVA for five independent samples. NS, no significance compared control group. *P<0.05 compared with NC group, ^#P<0.05 compared with NC+TGF-β1 group. (c) The RT-PCR analysis of the mRNA levels of p65, Col I, Col III, COX-2 and α-SMA expression in Fbs. The data are shown as the means±S.D. Statistical significance was calculated by one-way ANOVA for five independent samples. *P<0.05 compared with NC+TGF-β1 group. (d) Western blot analysis of the protein levels of p-p65, p65, Col I, Col III, COX-2 and α-SMA expression in Fbs. (e) Tcs cells (Tcs) proliferation. (f) The apoptosis of Fbs by flow cytometry. The data are shown as the means±S.D. Statistical significance was calculated using one-way ANOVA for five independent samples. ^$P<0.01 compared with NC group, ^&P<0.05 compared with NC+TGF-β1 group. (g) The RT-PCR analysis of the mRNA levels of p65, Col I, Col III, COX-2 and α-SMA expression in Tcs. The data are shown as the means±S.D. Statistical significance was calculated by one-way ANOVA for five independent samples. ^#P<0.05 compared with NC+TGF-β1 group. (h) Western blot analysis of the levels of p-p65, p65, Col I, Col III, COX-2 and α-SMA expression in Tcs